PDF(876 KB)
5-ALA-PDT联合放疗对常氧与乏氧人卵巢癌SKOV3细胞的杀伤作用
曹金发, 刘宏, 沈晶晶, 郭敏, 季可非, 熊康萍
中国医院药学杂志 ›› 2015, Vol. 35 ›› Issue (15) : 1397-1401.
PDF(876 KB)
PDF(876 KB)
5-ALA-PDT联合放疗对常氧与乏氧人卵巢癌SKOV3细胞的杀伤作用
Killing effects of 5-ALA-PDT combined with radiotherapy on human ovarian carcinoma SKOV3 cells under normoxia and hypoxia
目的:探讨5-氨基酮戊酸(5-ALA)介导的LED-光动力疗法(photodynamic therapy,PDT)联合放疗对人卵巢癌SKOV3细胞的杀伤作用。方法:常氧与乏氧下SKOV3细胞各实验组经不同浓度的5-ALA培养4 h后,由不同功率密度的635 nm红光和6 Gy X线照射,48 h后MTT法检测细胞存活率。结果:常氧与乏氧下0.1~0.7 mmol·L-1范围内,5-ALA无细胞毒性与放射增敏性(P>0.05);5-ALA>0.1 mmol·L-1,随着药物浓度与红光功率密度的增加,细胞存活率呈明显的下降趋势;X线与5-ALA-PDT联用,Ra×Rb/Rc比值分别为2.13、1.49,且常氧下联用与顺序无关,乏氧下有关(P<0.01)。结论:常氧与乏氧下X线与PDT联用都是协同作用,但乏氧下联用效果弱于常氧,且先X线照射后PDT可能会是一个更好的联用方式。
OBJECTIVE To study combined effects of radiotherapy and 5-aminolevulinic acid (5-ALA)-mediated LED-photodynamic therapy (PDT) on human ovarian carcinoma SKOV3 cells. METHODS SKOV3 cells were incubated with 5-ALA at different concentrations for 4 h and exposed to different fluence rates of 635 nm red light and 6 Gy X-ray radiation in independent treatment groups. After 48 h, percent cell survival rate was investigated using MTT assay. RESULTS 5-ALA had no cytotoxicity or radiosensitivity in range of 0.1-0.7 mmol·L-1 under normoxia or hypoxia (P>0.05). When 5-ALA concentration was over 0.1 mmol·L-1, SKOV3 cell survival rate showed a significant decreasing trend as 5-ALA concentration and red light fluence rates increased. Rates of Ra×Rb/Rc were 2.13 and 1.49 respectively under normoxia and hypoxia when X-ray combined with 5-ALA-PDT. Combination was not related with sequence under normoxia, but not under hypoxia (P<0.01). CONCLUSION X-ray combined with PDT has synergistic effects under normoxia and hypoxia, but effects of hypoxia were weaker than that of normoxia. It may be a better way for X-ray radiation following PDT.
放疗 / 光动力疗法 / 5-氨基酮戊酸 / 发光二极管 / SKOV3细胞 {{custom_keyword}} /
radiotherapy / photodynamic therapy / 5-aminolevulinic acid / LED / SKOV3 cells {{custom_keyword}} /
[1] Jemal A,Bray F,Center MM,et al.Global cancer statistics
[J].CA Cancer J Clin,2011,61(2):69-90.
[2] 孙建衡.妇科恶性肿瘤放射治疗学
[M].北京: 中国协和医科大学出版社,2002:204.
[3] 姜侃. 5-ALA-PDT对妇科恶性肿瘤细胞的杀伤作用及对SKOV3细胞HSP70、HO-1和HIF-1α表达的影响
[D].山东大学,2011.
[4] Luksiene Z,Kalvelyte A, Supino R. On the combination of photodynamic therapy with ionizing radiation
[J].J Photochem Photobiol B, 1999,52(1-3):35-42.
[5] Langland GT,Yannone SM,Langland RA,et al.Radiosensitivity profiles from a panel of ovarian caner cell lines exhibiting genetic alterations in p53 and disparate DNA-dependent protein kinase activities
[J].Oncol Rep,2010,23(4):1021-1026.
[6] Sazgarnia A, Montazerabadi AR, Bahreyni-Toosi MH,et al. In vitro survival of MCF-7 breast cancer cells following combined treatmentwith ionizing radiation and mitoxantrone-mediated photodynamic therapy
[J]. Photodiagnosis Photodyn Ther, 2013,10(1):72-78.
[7] Onuki J, Chen Y, Teixeira PC, et al. Mitochondrial and nuclear DNA damage induced by 5-aminolevulinic acid
[J]. Arch Biochem Biophys, 2004,432(2):178-187.
[8] Yamamoto J, Ogura S, Tanaka T,et al. Radiosensitizing effect of 5-aminolevulinic acid-induced protoporphyrin IXin glioma cells in vitro
[J]. Oncol Rep, 2012,27(6):1748-1752.
[9] 黄志勇,李步洪. 基于LED的光动力疗法光源设计
[J]. 激光与光电子学进展,2013,50(7):108-112.
[10] 宋梦姣,刘宏,葛宁,等. 甘氨双唑钠对人卵巢癌细胞株SKOV3的放射增敏效应
[J]. 中国医院药学杂志,2014,34(10):820-825.
[11] Duez P,Hanocq M,Dubois J. Photodynamic DNA damage mediated by delta-aminolevulinic acid-induced porphyrins
[J]. Carcinogenesis,2001,22(5):771-778.
[12] Ji Z,Yang G,Shahzidi S,et al. Induction of hypoxia-inducible factor-1alpha overexpression by cobaltchloride enhances cellular resistance to photodynamic therapy
[J]. Cancer Lett, 2006,244(2):182-189.
[13] Ding H, Yu H, Dong Y, et al. Photoactivation switch from type Ⅱ to type Ⅰ reactions by electron-richmicelles for improved photodynamic therapy of cancer cells under hypoxia
[J]. J Control Rel, 2011,156(3):276-280.
/
| 〈 |
|
〉 |
